The Total IgE ELISA kit is intended for quantitative determination of immunoglobulin E (IgE) in human serum.
Normally, IgE concentration in serum is very low. It increases gradually from birth to teen-age. In adults normal concentration of IgE may reach 100 IU/mL. In elderly people IgE level sometimes decreases.
IgE production is essential in anti-helminthic immunity. 15–20-fold increase in IgE concentration is observed at ascariasis.
However, in industrialized countries detection of high IgE concentrations is mainly linked with allergic diseases. Quantitative determination of total IgE has a great prognostic value. In 75% of children born from parents with allergic diseases serum IgE concentration is >95% from superior limit of normal range for corresponding age group. Detection of high IgE concentrations in serum by ELISA is an important tool for differentiation between allergic diseases and other pathologies with similar clinical manifestations (such as asthma, frequent respiratory diseases, chronic rhinites and dermatites).
Increased concentration of total IgE in serum is also reported in patients with lymphosarcoma and Hyper-IgE syndrome.
|Kit volume, wells (including controls)||96|
|Analytical sensitivity, IU/mL||2.3|
|Assay dynamic range, IU/mL||2.3- 500|
|Incubations||90 min + 10 min at 37°Cor
90 min at 37°C + 15-30 min at RT
|Shelf life, months||12|