The Free T3 ELISA kit is intended for quantitative determination of free triiodothyronine (free T3) in human serum.
Triiodothyronine (T3) is one of the thyroid gland hormones with a molecular mass of 651 Da.
L-Triiodothironine circulates in blood almost completely bound (>99,5%) to carrier proteins. However, only free triiodothyronine is thought to be responsible for the biological action. The free T3 concentration typically reflects the actual thyroid status of the patient more accurately than the total T3 concentration alone due to its independence of clinical conditions like pregnancy, oral contraceptives and steroid therapy.
|Kit volume, wells (including controls)||96|
|Analitical sensitivity, pmol/L||0.5|
|Precision: Intra Assay Variation, %||< 8|
|Range of evaluated concentrations, pmol/L||0.5- 60|
|Incubations||45 min + 15 min + 10 min at 37°Cor45 min + 15 min at 37°C + 15-30 min at RT|
|Shelf life, months||12|